Pawiser brand Rapid Bovine Brucella (B.Burcella) Ab Test Kit PW0301

INTENDED USE

The Bovine Brucella Antibody Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Bovine Brucella Antibody (B.Brucella Ab) in cattle’s serum or plasma specimen.

PRINCIPLE

The Bovine Brucella Antibody Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated Brucella LPS antigens. If there are anti-Brucella antibodies in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Bovine Brucella antibodies in the specimen.

KIT COMPONENT

-Test device with disposable dropper.

- Assay buffer

- Product Manual

STORAGE & SHELF LIFE

Store at 2-30°C, out of sun light and moisture.

Shelf Life: 24 months

TEST PROCEDURE

1.Allow all kit components and sample to reach room temperature prior to testing.

2.Collect the cattle’s fresh whole blood, serum or plasma specimen for use. Immediately apply the testing once collected the specimen.  

3.Take out the test device from the foil pouch and place it horizontally.

4.Add 20μL of serum or plasma specimen into an assay buffer tube. Mix well and directly use in the assay.

5.Add 2-3drops (approx.60-80μL) of assay buffer into the sample hole.

6. Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

INTERPRETATION OF RESULTS

Positive: Two lines appear, no matter T line is clear or vague.

Negative: Only C line appears.

Invalid: No line appears or only T line appears.

PRECAUTIONS

1.Do not use expired test or the test with damaged foil pouch.

2. Do not remove test cassette from its pouch until immediately before use.

3.If the serum cannot be tested immediately, store at 4℃ for short time (in 48hours), storeat below -20℃ for long-term storage.

4. Do not reuse the test kit and do not mix components from different lot numbers.

5. All specimens have potential infection. It must be strictly treated according to the rules by local states.

LIMITATION

Pawiser brand Bovine Brucella Antibody Rapid Test kit is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarian. For an accurate result, it is suggested to apply other method such as PCR for final determination in practice.