Pawiser brand Rapid CDV Ag Test Kit PW0002

INTENDED USE

Rapid Canine Distemper Virus Ag Test Kit is a sandwich lateral flow immunochromatographic assay for the qualitative detection of canine Distemper virus (CDV Ag) from dog’s secretions or serum.

PRINCIPLE

Rapid Canine Distemper Virus Ag Test Kit is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is enough Distemper antigen in the sample, a visible T line will appear. The C line should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Canine Distemper antigen in the sample.

KIT COMPONENT

- Test device, with disposable droppers

- Assay buffer  

- Cotton swabs

-Products manual

TEST PROCEDURE

1. Add 2 drops serum/plasma or 1 drop blood into the buffer tubes to agitate it.

2. If for secretions sample, use the swab to collect dog’s mucus or saliva and insert it into the buffer tube. Agitate it at least 10 times to get efficient sample extraction.

3. Take out the test device from the foil pouch and place it horizontally.

4. Gradually drip 5 drops of sample extraction into the sample hole with disposable droppers.

5. Interpret the result in 10-15 minutes. Result after 15 minutes is considered as invalid.

INTERPRETATION OF RESULTS

Positive: Two lines appear, no matter T line is clear or vague.

Negative: Only C line appears.

Invalid: No line appears or only T line appears.

PRECAUTIONS

1. The test must be used within 1 hour after opening the foil pouch.

2. Adding too much samples will cause false positive and overdosing assay buffer can also lead to incorrect results.

3. Extract samples should be tested immediately.

4. Do not use the expired test or the test with damaged foil pouch.

5. Do not reuse the test kit and do not mix components from different lot numbers.

LIMITATION

Pawiser Canine Distemper Virus Ag Test is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarian. For an accurate result, it is suggested to apply other method such as PCR for final determination in practice.